In order to determine pentosan in the malt,wort and beer accurately,orcinol-hydrochloric acid and phloroglucinol methods were used to compare accuracy and recovery.The results showed that Phloroglucdinol method could determine the content of pentosan in malt accurately and several varieties of malt identified are quite different from each other.Both methods do not directly measure wort,but when fermented by yeast,orcinol-hydrochloric acid method could determinate pentosan accurately.Orcinol-hydrochloric acid method can determine pentosan in beer accurately and big differences among several varieties of beer were also determined.