Biohydrogen production through dark fermentation has been a research focus,due to its high-capacity and high-speed of biohydrogen production,its sustainability and stability,simple operation of its reaction device and a wide range of low-cost renewable raw materials.In this paper,influencing factors including inoculum,temperature,pH and hydraulic retention time which are important to high efficient,continuous and stable hydrogen production by dark fermentation are summarized and the progress of biohydrogen production is introduced.