Aflatoxin contamination not only leads to serious economic losses,but also is detrimental to human and animal health.Much attention has been paid to mycotoxins biodegradation in recent years.For investigation of microbial detoxification,however,a reliable method is needed to decide whether the toxin is degraded or reversibly bound.In this paper,several methods were tested to differentiate the degrading AFB1 from adsorbing during the screening of aflatoxin B1-degrading microbes.Through comparing the AFB1 detoxification differences between living cells and dead cells,analyzing the residual AFB1 from microbial cells by aqueous washing and organic solvent extracting,and maintaining a stable pH of medium,we have effectively excluded the biological adsorbing and binding and pH interferences,and distinguished the degradation from the reversible adsorbing.It has been demonstrated that F4 and F7 strains have high AFB1 degradation activity.