The cAMP content is abundant and stable in jujubes,and is the highest in animals and plants tested so far.This paper used the method of ultrasound and water bath extraction to study the effect of three factors(time of ultrasound,concentration of alcohol,solid-liquid ratio) on the extraction rate of cAMP.The result showed that solid-liquid ratio was the main influencing factor,ultrasonic time,concentration of alcohol was then followed.The best extraction process of cAMP was : solid-liquid ratio 1∶ 25,solvent concentration15%,ultrasonic time 20min.The paper also used high performance liquid chromatographic to determine cAMP content in eight kinds jujubes,and it showed that chaoyangyuanzao had the highest(375.61μg/g) content,1-zaocuiwang had the lowest(46.01μg/g) content.