Peanut contains coenzyme Q10,the content of Q10 was detected by HPLC.The effect of different conditions by using ultrasonic cell-break method for extraction was systematically studied.The results showed that the size order of influence by four factors on the extraction rate was: extraction power > extraction time > duration of radiation each time > ratio of material to liquid.The power extraction and extraction time had significant effect on the extraction rate of coenzyme Q10.The optimal condition for coenzyme Q10 extraction was as follows: 400W,extraction time: 15min,duration of radiation: 6.0s/5,ratio of solid to liquid : 1∶ 6.Under this condition,the coenzyme Q10 extraction rate was 92.4ug/g.