The fermented broth of restructured E.coli as raw materials was used to extract glucosamine by ion exchange method.The effects of extraction conditions on glucosamine extraction were studied,and cation exchange resin 001 x 8 was selected.The affecting factors and dynamics properties of absorption of glucosamine by this resin were investigated.The optical conditions were: pH 2.2,temperature 30 ℃,elution with 0.7 mol/L of ammonium sulfate solution;Chromatography column Ф1 cm x 10 cm,sample amount 192 mL,injection velocity 3.19 BV/h,elution velocity 3.8 BV/h.Under those conditions,adsorption rate reached to 95% and the elution rate was up to 98.35%.