The paper reported the effects of the pressure,the dwell time and pH on the lipase activity,and focused on the changes of the lipase conformation through the Circular Dichroism(CD) spectroscopy and endogenous fluorescence spectroscopy.The main results were discussed as follows: In comparison with atmospheric pressure,the lipase activity under 200 MPa was increased by 32% at 70℃,pH7.5,15 min: With the extension of the dwell time,the lipase activity was stabilized,and the relative activities stayed above 120%.The optimal pH increased by 0.5.CD spectroscopy revealed that after high pressure treatment the secondary structure of lipase had changed,α-helix content increased significantly,β-sheet content decreased and endogenous fluorescence spectroscopy showed tryptophan characteristic peak rised by 10%～20%.