Antioxidant activities of polyphenol extracted from Rabdosia serra(Maxim.) Hara were studied.DPPH radical scavenging assay,H2O2 scavenging assay,reducing power assay,β-carotene bleaching assay and inhibition of linoleic acid peroxidation assay were conducted.The antioxidant activities of polyphenol from Rabdosia serra(Maxim.) Hara was compared with VC.Results showed that the antioxidant activities of polyphenol from Rabdosia serra(Maxim.) Hara were better than that of VC.The IC50 of polyphenol from Rabdosia serra(Maxim.) Hara on DPPH radical scavenging assay,H2O2 scavenging assay,β-carotene bleaching assay and inhibition of linoleic acid peroxidation assay were 5.00 μg/mL,42.51 μg/mL and 66.58 μg/mL respectively;the above assay on VC were 5.99 μg/mL,56.74 μg/mL and 94.83 μg/mL respectively.These results indicated that the antioxidant activities of polyphenol from Rabdosia serra(Maxim.) Hara were better than that of VC.