After royal jelly(RJ) was extracted with 95% ethanol and isolated with chromatographic columns MCI-Gel and ODS,adenosine monophosphate and 7 kinds of fatty acids were obtained.The scavenging free radicals(DPPH·)of 1,1-diphenyl-2-picrylhydrazyl and the total antioxidant capacity kit(T-AOC)were used to study antioxidation of alcohol soluble components.The results showed that antioxidation of royal jelly improved after extraction and the antioxidant activity of AMP and 3,10-dihydroxydecanoic acid(3,10-DDA)were relatively higher,IC50 value for DPPH· scavenging activity were 6.274 mg/mL and 9.153 mg/mL respectively.They were identified as the Antioxidants in royal jelly.