The synthetic UDP glycosyl-transferase UGT76G1 coding gene with modification was inserted into the vector pYES2 with the restriction site of EcoR I and Xho I to construct the recombinant Saccharomyces cerevisiae YPH499(pYES2–UGT) strain,which can express UDP glycosyl-transferase UGT76G1.A whole cell catalysis method for adjusting UDPG synthesis metabolic pathway of the yeast cell was established by providing glucose for carbon source and enhancing the UDPG flux with the substrate Stevioside.The optimal conditions for the whole cell catalysis system were as follows:1 g/L Stevioside,20 g/L glucose,10 g/L PluronicF68,6 g/L MgCl2,15 g/L citric acid sodium,pH 7.2,the cell density was 200 g wet cells /L reaction solution,the reaction temperature was 37 ℃,the reaction time was 72 h.Under the optimal conditions,the output of Rebaudioside A could reach 267.89 mg/L.