Food and Fermentation Industries >

2012 , Vol. 38 >Issue 07: 43 - 47

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.2012.07.022

Food and Fermentation Industries

Phylogenetic Diversity Analysis of Bacteria In Gujing-flavor Liquor Pit Mud Using Culture Independent Method

  • Tang Bin ,
  • Liu Jin-ying ,
  • Zhou Qing-qu ,
  • Li An-jun ,
  • Wan Chun-huan ,
  • Tang You-hong
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Online published: 2012-07-25

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Abstract

Culture independent method was used to study the diversity of Pit Mud bacteria.The techniques included culture independent approach,PCR technique and 16S rDNA sequence homology analysis etc.The total DNA directly extracted from Pit Mud was used as PCR template.Random clones containing almost full size 16S rDNA sequences(about 1.5 kb) were sequenced and subjected to an on line similarity search.According to the sequences alignment and homology analysis,the bacteria of Pit Mud were consisted of Uncultured bacterium,Clostridium,Lactobacillus,Eubacterium,and Syntrophomonas.

Key words： Gujing-flavor Liquor; Pit Mud; microbiology diversity; culture independent approach; phylogenetic analysis

Cite this article

Tang Bin , Liu Jin-ying , Zhou Qing-qu , Li An-jun , Wan Chun-huan , Tang You-hong . Phylogenetic Diversity Analysis of Bacteria In Gujing-flavor Liquor Pit Mud Using Culture Independent Method[J]. Food and Fermentation Industries, 2012 , 38(07) : 43 -47 . DOI: 10.13995/j.cnki.11-1802/ts.2012.07.022

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