This article first isolated spores from raw milk,and then applied the method of ultraviolet spectrophotometry to detect the contains of 2,6-dipicolinic acid,scaned between 385 nm to 265 nm and the first order derivative spectrum was obtained.Calculated the content of 2,6-dipicolinic acid by diffrence between the minimum and the maximum from 283 nm to 276 nm.Compared with the plate count,it showed a clear linear relationship between them,so the level of the spores in the raw milk can be assessed by the method of ultraviolet spectrophotometry.