Ganoderma lucidum strain was domesticated in Na2SeO3 medium to investigate its selenium(Se) accumulation ability.Atomic Fluorescence Spectrometry(AFS) and Liquid Chromatographic-Atomic Fluorescence Spectrometry(LC-UV-AFS) were used to determine the total Se contents and Se speciation in cultured Ganoderma lucidum,respectively.The results showed that the largest harvested biomass could reach 16.1 g/L and the respondent Se content could reach 13264 μg/g after the Ganoderma lucidum strain was domesticated by Na2SeO3 medium,which were significantly better than those in non-domesticated ones.SeMet,SeCys2,SeMeCys and inorganic selenium(Se(IV)) could be determined in cultured Ganoderma lucidum,and SeMet and SeCys2 fractions could be 60%~90% and 10%~40%,respectively.It should be pointed out that the Se species was dominated by Se(IV) instead of Se-amino acid if only the contents of Na2SeO3 was increased.