This study was designed to explore freezing injury and recovery mechanism of Aeromonas spp.,one of specific spoilage bacteria,and experiments based on bacterial cell membrane and cytoplasm were carried out.After Aeromonas spp.was treated for 24 h at-18℃,variations of 260 nm and 280 nm absorbance of supernatant from cell centrifugation were measured,and also the inhibition of nucleic acids,peptidoglycan and ATP were studied.The results indicated that the permeability of cell membrane of Aeromonas spp.was damaged,thus leading it sensitive for 1.5% NaCl.Moreover,the synthesis of DNA and peptidoglycan were not destroyed,but the synthesis of RNA and ATP were inhibited,which might be possible injury mechanism of Aeromonas spp..