Steviol glycosides have been used widely as natural intensive sweeteners.The transglycosylation of stevioside with cornstarch hydrolysate catalyzed was investigated using a new α-cyclodextrin glucanotransferase from Paneibacillus macerans JFB05-01.Optimum conditions were as fellows: 60 ℃,DI water as solvent,cornstarch hydrolysate 15mg/mL and stevioside 10mg/mL,enzyme loading 15 U/g St,3h,final conversion of stevioside and yield of St-Glu 1 were 59.2% and 32.4%,respectively.The hydrolysis of transglycosylated products with glucoamylase increased St-Glu 1 to 43.1%.Solubility of glycosylated derivatives was 60 times as high as that of stevioside,whose taste was improved as well.