Food and Fermentation Industries

LAMP (Loop-mediated Isothermal Amplification) Detection of Genetically Modified Soybean Products

  • Ye Lei ,
  • Shen Hui-ping ,
  • Yan He ,
  • Li Lin ,
  • Shi Lei
Expand

Online published: 2012-08-25

Abstract

Specific LAMP primers were designed on the basis of published sequence of lectin gene,NOS terminator and two GM soybean strain GTS40-3-2 and MON89788.CTAB DNA extraction method was used to extract DNA of soybean products from local supermarkets.Conventional PCR and LAMP assays were conducted to verify the accuracy of the LAMP method.The results showed that the LAMP assays with the primers designed in the study are specific and sensitive.Traditional CTAB method can successfully extract DNA from soybean products and the DNA purity is suitable for nucleic acid amplification.The PCR results were consistent with LAMP results.This indicated that LAMP method is very accurate.

Cite this article

Ye Lei , Shen Hui-ping , Yan He , Li Lin , Shi Lei . LAMP (Loop-mediated Isothermal Amplification) Detection of Genetically Modified Soybean Products[J]. Food and Fermentation Industries, 2012 , 38(08) : 150 -156 . DOI: 10.13995/j.cnki.11-1802/ts.2012.08.045

Outlines

/