To obtain strains with positive activity of transforming ginsenoside from Panax notoginseng materials sampled in Wenshan of Yunnan.Methods: Using plate streaking method,two fungi were isolated from samples of Panax notoginseng roots.The samples of Panax notoginseng were fermented with the fungi,and the changes of saponins were determined by HPLC methods.Morphologic identification and ITS sequence analysis of the two fungi were carried out.Results: The two strains should belong to the genus Trichoderma based on the comparison and analysis of its morphology and characteristics of colony and sporulation structure.The ITS length of these strains were 550bp,and only one deletion mutation occasionally was taken place at the site 134 of ITS.It was indicated that these strains were highly similar to other strains of Trichoderma longibrachiatum Rifai(ATCC18648T,ATCC208859,DAOM167674,DAOM231259).So these two strains were identified as T.longibrachiatum.Results from HPLC showed that the strains could efficiently convert big polarity ginseng saponins into small polarity ginseng saponins with high yielding ginsenoside Rd.Conclusion: T.longibrachiatum should possess activity for transforming ginseng saponins.