Use flue gas derived from a methanol plant which contains high concentration of carbon dioxide to cultivate Chlorella sp.,the effects of different aeration modes and cultivation modes on the growth of Chlorella sp.were determined.The results indicated that the microalgae biomass increased when flow rate was at 300 mL/min of 10% CO2 per min and the flow frequency was 10 min/h,the microalgae dry mass and biomass productivity were 0.716g/L and 0.088 gg/(L·d),respectively.The biomass concentration obtained in fed-batch cultivation with nitrate and phosphorus feeding was higher than that obtained in batch cultivation,the maximize microalgae dry mass and biomass productivity were 0.746g/L and 0.093g/(L·d).Respectively.The higher microalgae biomass was obtained when the renewal rate was 20%.When mixotrophic culture with acetic acid,while acetic acid(concentration from 50μL/L to 125μL/L) was added to the culture medium,it could accelerate the growth of Chlorella sp..When acetic acid(concentration from 10μL/L to 25μL/L)was added to the culture medium daily,higher growth rates were obtained than that in normal conditions,and the maximize microalgae dry mass and biomass productivity were 0.986g/L and 0.119g/(L·d),respectively,which were 1.3 times and 1.4 times higher than that of the controls.The study shows that using flue gas derived from a methanol plant to enhance the microalgae biomass of Chlorella sp.is feasible.