Polygonatum glycoprotein was extracted from polygonatum by hot water and sinking in alcohol.The trichloroacetic acid,the dialysis and the anion-exchange column chromatography were used to purify the Polygonatum glycoprotein.Monosaccharide composition of polygonatum glycoprotein was studied by GC-MS and the antioxidant activity of Polygonatum glycoprotein were studied by Fenton oxidation,phosphomolybdenum complex method and pyrogallol autoxidation method.The results showed that: The content of Polygonatum glycoprotein was 0.34%,the ratio of polysaccharide and protein was 5.4∶ 1,the monosaccharide composition of Polygonatum glycoprotein mainly were rhamnose,arabinose,D-xylose,galactose,glucose and mannose polysugar and their relative percentage contents were: 2.12%,1.81%,2.51%,65.4%,14.3%,13.8%.Scavenging ratio of hydroxyl radical,scavenging ratio of superoxide anion radical and total antioxidant activity of polygonatum glycoprotein increased gradually by the contents of polygonatum glycoprotein.Scavenging ability of hydroxyl radical and superoxide anion radical were higher than that of Vc,however the total antioxidant activity was lower than that of Vc.