A method for the determination of acrylamide in Chinese traditional fermented bean products by HPLC-MS was established.ACQUITY UPLC C18 column(210mm×20mm,1.7μm) was used for chromatographic separation,with elution of 0.1% formic acid in water and methanol(99∶ 1 by volume).Determination was performed by using electrospray ionixation(ESI+) and multi-reaction monitoring(MRM).Internal standard method was applied to quantify.The m/z for acrylamide was 72.2∶ 55,the m/z for 13C3-acrylamide was 74.79∶ 58.0.The calibration linear range of acrylamide was from 0.01~1μg/mL with correlation coefficient of 0.992425.The mean recovery of fermented bean curd,fermented black bean and soybean paste were 86.6%,91.7% and 85.0%,the relative standard deviation was lower than 10%(n=5).This method was highly sensitive and well repeatable,and was suitable for quantifying the contents of acrylamide in Chinese traditional fermented bean products.