In this paper,SDS-PAGE electrophoresis and fluorescence spectroscopy were used to analysis Chinese date honey(CDH) protein.It is shown that there were significant differences on protein content of CDH from different areas.For example,the protein content of CDH in Jiaxian was the highest(1468.18 μg/g),whereas that in Fuping was the lowest(719.03 μg/g).The main protein bands of CDH in different areas in Shaanxi were little difference.However,the protein bands of CDH from different province showed highly significant difference,with specific protein bands.Meanwhile,the maximum peak and intensity of CDH protein fluorescence from difference areas have significant differences,indicating significant differences on protein composition and structure of CDH.