A pre-column derivatization high-performance liquid chromatography(HPLC) method for the determination of taurine in Takifugu obscurus was developed.Taurine was derivatized with Ophthalaldehyde(OPA).The derivatives were separated on a reversed-phase XbridgeTM C18 column by a mobile phase by(A) methanol,(B) acetonitrile and(C) water(10/20/70).Sample size was about 20 μL,flow rate was about 1 mL/min and detection was done at 330 nm by ultraviolet detector.Results showed that the linear ranger of taurine was 5~30μg/mL,correlation coefficient R2=0.9991,the recoveries were 95.35%~98.19%,RSD=2.03~3.71%(n=3).The precision RSD = 0.51%(n=6) and the detection limit was1.14μg/mL.The method is simple,quick,specific,sensitive,repetitively and accurate.