In the present study,we cloned the tfu gene encoding cutinase from Thermobifida fusca.To accomplish the secretion of cutinase,α-factor signal peptide was added at the N terminal of cutinase.After successfully construction of the recombinant strain,the effects of concentration of galactose and induction mode were deeply analyzed.Moreover,we investigated the effects of different combinations of mixture of carbon source on the production of cutinase.As a result,raffinose was proved to be much more active than other sugars applied for overexpression of cutinase.The results achieved herein would provide an alternative for cutinase production and its potential application.This would be more attractive in the food and pharmaceutical industry.