A new method for purification of steviol glycosides from Stevia Rebaudiana crude extracts by macroporous adsorption resin(MAR) was systematically investigated.The purification procedure consisted of two steps.In the first step,the main purpose was decolorization,and several types of macroporous resins including D293,D296,JN-1,JN-2,D890 and D201 were used in order to choose the best resin.JN-1 resin was selected according to their decolorization ratio,the retention of steviol glycosides ratio and desorption ratio.In order to increase the purity,a second step of refining was added by screening various types of macroporous resins(AB-8,JNF-1,X-5,NKA-II and D101).The results demonstrated that JNF-1 resin was the most suitable resin.The purity of steviol glycosides after the first step JN-1 was from 42.65% to 67.33% with a recovery of 84.87%.The purity of steviol glycosides increased to 92.92% with a recovery of 91.08% after the second step JNF-1.