When the concentration of chitosan solution was 2.0 %,the composition of coagulation liquid was 15%NaOH∶ 75%ethanol = 4∶ 1(in Volume),and it was treated with 5.0% glutaraldehyde for 6 hours,globular cross-linked chitosan carrier was made.The application of pectinase immobilized on globular cross-linked chitosan in clarification of loquat juice was studied.The results showed that the process conditions were as follows: amount of immobilized enzyme given for 50 g/L juice,pH value for 3.0,concentration of loquat juice for 40%,and 50 ℃ for 1.5 hour.The transmittancy of loquat juice was 75.8% after immobilized enzyme was repeatedly used seven times.The clarification effects of immobilized enzyme were better than that of free enzyme.Using immobilized enzyme to hydrolyze the pectin of juice,the sugar content and titratable acidity had no change.The separation between juice and sediment became easier.The color of loquat juice become thin yellow,and showed higher stability to cold and heat.