By A broad spectrum bacteriocin producing strain was screened by plant punching and its antibacterial characteristics was studied.The results showed that one bacteriocin-producing Lactic acid bacteria strain isolated from plant materials exhibited strong inhibition activity against the E.coil.The supernatant of this strain could still inhibit the growth of indicator strain strongly after eliminating hydrogen peroxide and organic acid.After treatment with pepsin,the strain's inhibitory activity decreased sharply,which showed the inhibitory substance possessed the characteristic of protein,and it could be named bacteriocin.The strain was identified as Lactobacillus plantarum through detection of its appearance,physiological and biochemical characteristics.The inhibitory activity of strain fermentation supernatant was not affected by high temperature,1% Tween-80,SDS,EDTA and the low pH.However,its inhibitory activity revealed an obvious decrease after treatment with protease K and trypsin than with pepsin.The bacterial inhibition spectrum showed that this was a kind of bacteriocin with wide inhibition spectrum.