This paper introduces a new content determination method of total flavonoids in propolis products.The test solution goes through polyamide powder absorption and vacuum extraction-filtering treatment,then,with sodium nitrite-aluminium nitrite-sodium hydroxide as the chromogenic agent and rutin the reference substance,detects the total flavonoids in propolis at a wavelength of 510 nm by spectrophotometry.T test analysis shows that there is no significant difference between this method and the method of National Standard ,and it is proved to be highly accurate and precise.This method is more suitable for determination of total flavonoids in propolis capsules and other lipophilic samples.