Using Aspergillus niger as the original strain,a high throughput method was established to screen calcium gluconate high producing strains with 48-well plates.From the spores dealed with UV-LiCl complex mutation,4 high-product strains was obtained by high throughput screening,whose fermentation efficiency was excellent after the passages of strains.According to high product of 23.04 g/100 mL for the strain of P-9,the yield was increased by 5.86 g/mL.Comparing with the traditional shake flask screening platform,this method could significantly improve the screening efficiency of calcium gluconate high producing strains.