The influence factors of oat β-glucan on the adsorption of epigallocatechin gallate(EGCG) in vitro simulated gastrointestinal fluid were studied.The result showed that the oat β-glucan adsorption EGCG was more likely influenced by pepsin,trypsin,pH and EGCG concentration.The amount of EGCG adsorption increased if pepsin or trypsin was added to vitro simulated gastrointestinal fluid.The adsorption capacity of the β-glucan gradually increased with pH increasing in the simulated gastric fluid.In intestinal fluid,the capacity increased first and then declined with the pH increasing.The biggest adsorption capacity was at pH 6.5.With EGCG concentration increased,the response factors increased,so did the adsorption capacity of the β-glucan.