A HPLC method for rapid determination of four organic acids in Jiangshui was developed in this paper.Agilent ZORBAX SB-AQ column(250 mm×4.6 mm,5μm) was used at 30℃.The mobile phase was CH3OH∶ 0.01 mol/L KH2PO4(0.5∶ 99.5,pH=2.0).The flow rate was 0.6 mL/min and the detection wavelength was set at 210 nm.The injection volumes were 20 μL for each run.Based on the above optimizing chromatogram conditions,four organic acids,including tartaric acid,lactic acid,acetic acid and fumaric acid,were separated satisfactorily.The intra-day and inter-day precision(RSD%) for the analytes ranged from 0.6% to 1.8% and from 1.7% to 4.2%,respectively.The average recovery factors were from 81.0% to 116.0%.The results showed that developed method was successfully applied for the detection and isolation of four organic acids in Jiangshui.