Different proportion of leaves residual of Eucommia ulmoides oliv were used to cultivate Pleurotus and its effects on the time of full bottle of mycelium,growth rate,growth vigor of mycelium,contents of general flavonoids,protein,fat and related trace elements of Pleurotus were studied.The results showed that when 10%~40% cotton seed hull were replaced by Eucommia ulmoides oliv leaves residual,the time of full bottle of mycelium was the shortest,the growth rate of mycelium was the fastest and the growth vigor of mycelium was the best.When 100% cotton seed hull was replaced,the content of general flavonoids was the highest,which was 3.72 times than the control group.When 70% cotton seed hull was replaced,the content of protein was the highest,which was 1.04 times of the control group.When 40% cotton seed hull was substituted,the content of fat was the lowest,which was 0.660 times of the control group.When 60% cotton seed hull was superseded,the content of iron,zinc,sodium and potassium were the highest,which were 2.47,1.82,1.85,1.27 times of the control group.When 60% cotton seed hull was replaced,the content of calcium,magnesium,manganese and copper were the highest,which were 1.68,1.80,1.79,1.58 times of the control group.