Fatty acid in blueberry fruit was extracted by Soxhlet and its methyl ester product was analyzed by GC-MS.The fatty acid composition and changes of blueberry fruit during cold storage was determined by peak area normalization method and data processing systems of MSD chemistry workstation.Results indicated that there were eight fatty acids in blueberry.The major components were linoleic acid(41.874%),palmitic acid(27.343%),linolenic acid(14.680%),oleic acid(9.285%),arachidic acid(1.437%),13-octadecenoic acid(1.204%),hexadecenoic acid(0.555%) and stearic acid(0.411%).Linoleic acid,linolenic acid and oleic acid were the major components of unsaturated fatty acids and their relative percentage content was 67.598%.During the cold storage at 0 ℃,the composition of fatty acid had not changed,but the content of unsaturated fatty acid decreased gradually and the saturated fatty acid increased.The content of unsaturated fatty acid decreased significantly after 45d.The index of unsaturated fatty acids and unsaturation degree both decreased during cold storage.