We have analyzed and compared the antioxidant activity of liquid fermentation mycelium of five kinds of edible fungi.These edible fungi included Hericium erinaceus(Bull.) Per.,Pleurotus eryngii Quel.,Lentinus edodes(Berk.) sing.,Flammulina velutiper(Fr.) Sing,and Copyinds comatus(MUII.Fr) Gray.We have extracted the fermentation mycelium polysaccharide and protein of five edible fungi and determined their antioxidant activity.Antioxidant activity was evaluated by four methods,including the measure of reducing power and the evaluation of capacities of scavenging for superoxide anion free radical,hydroxy radical,and DPPH free radical.We also have studied the effect of the polysaccharides and protein content on the antioxidant activity.The results showed that the fermentation mycelium polysaccharide and protein had some reducing power and scavenging capacity to O· 2-,·OH and DPPH·.A comprehensive evaluation method had been established to evaluate the composite antioxidant capacity of fermentation mycelium polysaccharide and protein of five edible fungi.The results showed that the antioxidant activity of Flammulina velutiper(Fr.) Sing mycelium polysaccharide was the highest in the five mycelium polysaccharides.The order of antioxidant activity of mycelium polysaccharide from high to low was as followes: Flammulina velutiper(Fr.) Sing,Hericium erinaceus(Bull.) Per.,Pleurotus eryngii Quel.,Lentinus edodes(Berk.) Sing.,Copyinds comatus(MUII.Fr) Gray.Antioxidant activity of Hericium erinaceus mycelium protein was the highest in the five mycelium proteins.The order of antioxidant activity of mycelium protein from high to low was as followes: Hericium erinaceus(Bull.) Per.,Flammulina velutiper(Fr.) Sing,Pleurotus eryngii Quel.,Lentinus edodes(Berk.) Sing.,Copyinds comatus(MUII.Fr) Gray).Determination results of mycelia polysaccharide and protein content showed that the higher content of polysaccharide and protein,the stronger comprehensive antioxidant activity.