For the first time,the extraction optimization of total flavonoids in iris dichotoma optimized by orthogonal experiments is reported.The results showed that the order of the factors influencing the optimum extraction technology was: ethanol concentration > extraction temperature > ratio of solid to liquid > extraction time.The best extraction technology conditions was A 2 B 2 C 3 D 1,80% ethanol concentration,ratio of solid to liquid 1∶ 20,extraction time 5 min,extraction temperature 90℃,the total flavonoids was 4.007 mg / g.The experiment provided scientific basis of the development and utilization of iris dichotoma flavonoids.