To improve the production of ε-poly-L-lysine(ε-PL) by Streptomyces sp.M-Z18,the strategy of Lglutamic acid feeding during fermentation was developed with the help of optimization of L-glutamic acid supplement concentration and time.Combined with fed-batch fermentation using glycerol as carbon source,the ε-PL production and productivity was reached to 31.65 g / L and 4.36 g /(L·d) respectively in 174 h.Compared with the fermentation without L-glutamic acid addition,the data were increased by 49.2% and 43.9%,respectively.These results demonstrated that the feeding of L-glutamic acid during fermentation was a novel approach for improve ε-PL fermentation level by ε-PL producing strains.
DONG Nan
,
CHEN Xu-sheng
,
REN Xi-dong
,
ZENG Xin
,
SUN Qi-xing
,
ZHANG Jian-hua
,
MAO Zhong-gui
. Enhancement of ε-poly-L-lysine production through L-glutamic acid feeding during fermentation by Streptomyces sp. M-Z18[J]. Food and Fermentation Industries, 2013
, 39(07)
: 79
-82
.
DOI: 10.13995/j.cnki.11-1802/ts.2013.07.014
