The effects of tea polyphones(TP),dihydromyricetin(DMY),rosemary,Vitamin E(V E) and two synergists(ascorbic acid and citric acid) on the oxidative stability of the Paeonia seed oil extracted by supercritical CO 2 were studied by Schaal experiment.Using peroxide value(POV) and thathiobarbituric acid(TBA) as the evaluation index,the results showed that 0.02% TP and 0.02% DMY exhibited greater anti-oxidative effect than others,and TP with ascorbic acid(V C) or critic acid(CA) had great synergistic effect,and the effect of V C is superior to that of CA.0.01% TP with 0.01% DMY and 0.01% V C had remarkable anti-oxidative effect on the oil.The shelf time of the oil could be prolonged from 1.4 months to 12 months in 20℃ by the anti-oxidant complex.