In order to further utilization of Ulva fasciata polysaccharide,based on the single factor test,response surface method( RSM) was employed to obtain the best result of polysaccharide extraction rate. The physico-chemical properties of the crude polysaccharide were analyzed. and the effects of the sensory pleasurableness of cigarette smoke were investigateadd after add it to cigarette. The results showed that the optimal conditions for the extraction of Ulva fasciata polysaccharide with ultrasonic-assisted were as follows: extractiontime of 33 min,ultrasonic power of 620W,pulse recurrent time of 0 s,polysaccharide extraction rate up to 14. 20%. The total sugar of crude polysaccharide is38. 63%,uronic acid content is 35. 06%,sulfate radical content is 19. 41%,protein content is 0. 28%. The results of sensory evaluation showed that Ulva fasciata polysaccharide could increase the aroma quantity,improve the smoothness and sweetness,and decrease offensive odor of cigarette smoke.