The effect and mechanism of pulsed electric field( PEF) treatment on the chelation reaction between glycine and copper sulphate was investigated. The optimum reaction condition without PEF treatment was pH 7. 0,at50 ℃,the chelation ratio of glycine to copper was 2: 1. PEF treatment over 20 kV / cm led to a noticeable increase in the yield of biscopper in a certain period of time. It was found that 89% increase of the biscopper yield by PEF at 40kV / cm for 50 min. Fourier transform infrared spectroscopy( FTIR) analysis showed that the chelate prepared by PEF treatment was much more stable than the untreated one. In conclusion,PEF could be an alternative method to enhance the chelation reaction.