This study proposed a three-stage technology of simultaneous saccharification and co-fermentation( SSCF). After rice straw exploded by 0. 9 mol / L butanone solution,the mixed fermentation using Saccharomyces cerevisiae( S. cerevisiae) and Candida shehatae( C. shehatae) as mixed strains for producing ethanol were optimized and amplified. The fermentation was performed in 30 L ventilated fermentor under the conditions optimized by shake-flask fermentation,and parameters were set as the following: the dry matter content of rice straw was set at 8%,cellulase loading at 15 U / g dry matter,β-glucosidase loading at 3. 5 U / g dry matter,xylanase loading at 12 U / g dry matter,enzymatic hydrolysis( EH) at 50 ℃ for 25. 1 h,inoculum concentration of S. cerevisiae starter at 8%,first fermentation( S. cerevisiae) at 37 ℃ for 34. 1 h,inoculum concentration of C. shehatae at 7. 8%,second fermentation( mixed fermentation) at 35 ℃ for 6 d,and air volume of the mixed fermentation at 0. 3 L/( min·L). Under above conditions,the ethanol yield was 26. 9 g /100g rice straw dry matter. In this process,the utilization rates of glucose and xylose of rice straw were 91. 4 and 84. 7%,respectively. The study may offer a reference for further SSCF application and study on converting lignocellulosic material to ethanol without supplementing nutrients.