Three natural crude extracts of β-glucan from hulless barley,oat and yeast were used as the study objects and the synthetic polydextrose was used as the control. We conduct seven experiments on functional property in vitro,including the adsorption of oil cholesterol cholate and glucose,the inhibition of pancrelipase and amylopsin,and the cation exchange. The result shows,one gram of crude extract of β-glucan from hulless barley can adsorb6. 28g corn oil,20. 49mg cholesterol,18. 91mmol glucose,and the capacity of inhibiting pancrelipase and amylopsin is 28. 1% and 92. 9%,respectively. The extracts from hulless barley has comprehensive advantages on lowering blood pressure and adjusting blood glucose and is the best among four samples. The crude extracts of β-glucan from oat and yeast have roughly the equal adsorption of cholate,and their adsorption capacity is significantly higher than the crude extract of β-glucan from hulless barley. The cation exchange capacity of crude extract of β-glucan from yeast is the highest of four samples. The polydextrose also has the seven functional properties in vitro,but it doesn't show any competitive advantages.