In this study,the preparation,regeneration and inactivated condition of yeast protoplast were optimized on the basis of constructing a haploid cells parental library of wine yeast and malic acid degradation yeast. Then,a fusant,named F8,was isolated by preliminary screening of malic acid degradation indicator plates and second screening of grape juice fermentation through two rounds genome shuffling. The total acid and alcoholicity of grape juice fermentation liquor were 4. 89 g / L and 10. 39%( v / v),which were similar to that of parental strain. This study successfully proved that the effective and efficient of genome shuffling in the wine yeast breeding,and laid the foundation for the wine production that brewing wine and reducing acid synchronously.
LI Wei
,
ZHU Yue-lian
,
YI Ju-yang
,
HUANG Mei-hua
,
ZHANG Jin
,
CHEN Guo-pin
,
CAO Mu-ming
,
ZHANG Ying
. Breeding wine yeast with strong deacidification ability by genome shuffling and its fermentation characteristics[J]. Food and Fermentation Industries, 2014
, 40(02)
: 89
-93
.
DOI: 10.13995/j.cnki.11-1802/ts.2014.02.035
