The mycelia were acquired by liquid submerged fermentation with Hirsutella sinensis. The extraction conditions were optimized through orthogonal test as follows: the extraction temperature was 100 ℃,the extraction time was120 min,and the ratio of solid to liquid was 1∶ 15,for four times extraction. The crude polysaccharide was stepwise purified by Sevag deproteinization,dialysis,DEAE cellulose Ion exchange and Sephadex G-100 column chromatography,and three kinds of polysaccharides( named HSP-1,HSP-2,and HSP-3) were obtained. After ultraviolet scanning and Sephacryl S-300 HR column determination,HSP-1 and HSP-2 were regarded as homogeneous components while HSP-3 was inhomogeneous. The results from Phenol-sulfuric acid determination showed that the contents of HSP-1 and HSP-2 were 89. 42% and 85. 63%,respectively. The average molecular weights of HSP-1 and HSP-2,which were determined by Sephacryl S-300 HR column chromatography,were estimated to be 1. 7 × 104Da and 9. 8 × 103Da,respectively. The results of GC-MS showed that monosaccharide compositions of HSP-1 contained D-glucose,D-mannose and D-galactose with molar ratio of 4. 522 ∶ 1 ∶ 1. 378,while monosaccharide compositions of HSP-2 were D-glucose,D-mannose,D-galactose and D-arabinose with molar ratio of 2. 687∶ 4. 591∶ 1∶ 0. 2121. The FT-IR detection showed that the α-configuration glycosylic bond was contained in both HSP-1and HSP-2.