Food and Fermentation Industries

Analysis of lactic acid bacteria in traditional dairy products by culture-independent and denaturing gradient gel electrophoresis(DGGE) methods

  • DONG Xiao-wan ,
  • LI Bao-kun ,
  • LU Shi-ling ,
  • CHEN Qing-ming ,
  • LU You-guo ,
  • CHANG Han-ze
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Online published: 2014-03-25

Abstract

In order to analyze lactic acid bacteria community structure in traditional dairy products and explore the predominant microbial species,both culture-dependent and culture-independent methods and denaturing gradient gel electrophoresis( DGGE) based on the sequence of 16S rDNA V6-V8 region gene were used to examine the total lactic acid bacteria counts. The results showed that 5 kinds of lactic acid bacteria were identified from traditional dairy products by the Culture-independent method,wherein the dominant bacteria belonged to Pediococcus acidilactici. By 16S rDNA V6-V8 region gene DGGE method,the most dominant bacteria was Lactobacillus helveticus,the second is Pediococcus acidilactici. This suggested that Independent culture combined with PCR-DGGE could be applied to analyze bacterial community structure and predominant bacteria in traditional dairy products effectively,thus provide a theoretical reference for the development of culture starter and that will give us more information of microorganism diversity.

Cite this article

DONG Xiao-wan , LI Bao-kun , LU Shi-ling , CHEN Qing-ming , LU You-guo , CHANG Han-ze . Analysis of lactic acid bacteria in traditional dairy products by culture-independent and denaturing gradient gel electrophoresis(DGGE) methods[J]. Food and Fermentation Industries, 2014 , 40(03) : 97 -101 . DOI: 10.13995/j.cnki.11-1802/ts.2014.03.027

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