In order to analyze lactic acid bacteria community structure in traditional dairy products and explore the predominant microbial species,both culture-dependent and culture-independent methods and denaturing gradient gel electrophoresis( DGGE) based on the sequence of 16S rDNA V6-V8 region gene were used to examine the total lactic acid bacteria counts. The results showed that 5 kinds of lactic acid bacteria were identified from traditional dairy products by the Culture-independent method,wherein the dominant bacteria belonged to Pediococcus acidilactici. By 16S rDNA V6-V8 region gene DGGE method,the most dominant bacteria was Lactobacillus helveticus,the second is Pediococcus acidilactici. This suggested that Independent culture combined with PCR-DGGE could be applied to analyze bacterial community structure and predominant bacteria in traditional dairy products effectively,thus provide a theoretical reference for the development of culture starter and that will give us more information of microorganism diversity.
DONG Xiao-wan
,
LI Bao-kun
,
LU Shi-ling
,
CHEN Qing-ming
,
LU You-guo
,
CHANG Han-ze
. Analysis of lactic acid bacteria in traditional dairy products by culture-independent and denaturing gradient gel electrophoresis(DGGE) methods[J]. Food and Fermentation Industries, 2014
, 40(03)
: 97
-101
.
DOI: 10.13995/j.cnki.11-1802/ts.2014.03.027