In this study,51 strains of lactic acid bacteria were isolated from Bama longevity population gut. Among them,16 strains were selected for their abilities to lower cholesterol and riglycerids with lowering rates of more than 30. 00% and 5. 00%,respectively. The Lr10 was significantly effective than others( P < 0. 05),in which the lowering rates reached 61. 90% and 37. 22%,respectively. The Lr10 had strong ability of bile-salt and low pH tolerance. Its survival rates were 71. 43%,66. 37%,64. 55% and 19. 12% in pH 3. 0 simulated gastric juice and MRSbroth with 0. 3%,0. 5%,and 1. 0% bile salt,respectively. It was identified as Lactobacillus rhamnosus by API and 16s rDNA sequence alignment. The Lr10 was prepared into skim milk suspension and skim fermented milk to test its lipid-lowering ability in vivo,the result showed that Lr10 and its fermented milk had a remarkable lipid-lowering effect in vivo.