To improve the low temperature protease activity of Serratia sp. A29-2 isolated from Xinjiang glaciers, the fermentation medium and fermentation conditions of Serratia sp. A29-2 were optimized respectively through orthogonal experiment and response surface test on the basis of single factor test. The results showed that the optimum culture media contained peptone 15. 0g / L,yeast extract 5. 0g / L,maltose 10 g / L,CaCl20. 5g / L. The optimum fermentation conditions were temperature 23℃,pH 7. 0,fermentation time 60h,mass fraction of NaCl 1. 0%. Under the optimum conditions,the low temperature protease activity produced by Serratia sp. A29-2 reached 54. 25U / mL,which was 1. 93 times higher than that under the condition before optimization.
WU Yun
,
ZHANG Xiao-yan
,
ZHOU Jian-zhong
,
YIN Ming-yuan
,
WANG Xiao-biao
,
MIAO Sen
,
HAN Yan-peng
,
MA Rui
. Optimized fermentation conditions of the low temperature protease production using strain Serratia sp.A_(29)-2[J]. Food and Fermentation Industries, 2014
, 40(04)
: 71
-76
.
DOI: 10.13995/j.cnki.11-1802/ts.2014.04.016
