We selected the best macroporous resin through static adsorption and obtained the optimal purification conditions by the dynamic desorption. The results were as follows: X-5 macroporous resin exhibited the excellent characteristics for purification of Solanum nigrum L. anthocyanins. We found that the time of absorption and desorption equilibrium was 5 h and 6 h,respectively; the column diameter to length ratio was 1∶ 25,the maximum sample volume was 0. 6 BV,the concentration of absorption equilibrium was 0. 4 mg / mL,the eluent volume was 3. 5 BV, and elution flow rate was 2. 0 BV / h,60 % ethanol with pH 2. 0 was used as eluent. Under those optimal conditions, the purity was 19 times higher of unpurified ones. This research provides a theoretical base for deep processing of Solanum nigrum L. berries