In order to meet the requirement of nano-filtration membrane,a integrated process consisting of coagulate-activated charcoal-ultrafiltration( UF) was proposed to treat pullulan fermentation broth. The results demonstrated that the optimum dosage of coagulate was 10 g /L and the optimum dosage of activated charcoalwas was 25 g /L,the decoloration time was 65min. In the experiment,the polysulfone ultrafiltration membrane with molecular weight cut-off67 kDa had a better effect on concentrating pullulan. The optimum technological parameters of polysulfone ultrafiltration were: the operation pressure 0. 08 MPa and the temperature of fermentation broth 298. 15k,the pH of fermentation broth 3. 5. The concentration of fermentation broth was from 10 g /L to 27 g /L and the recovery rate of pullulan was up to 85%.