Purification process of Macroporous resin for apple polyphenols was studied. XAD7HP macroporous resin showed the best adsorption and desorption capacity among six kinds of macroporous resins. On the basis of single factor optimization,an orthogonal experiment was designed to optimize the purification process. Results showed that the optimal purification conditions were: sample concentration 1. 46 mg /ml,sample pH 5. 50,the flow rate of sample1. 25 ml /min,eluant type 90% acetone,eluent flow rate 1 ml /min. Under the above conditions,purity was up to80. 1%,10 times more than before. The recovery rate was as high as 90%. Purified Apple polyphenols were analyzed by High Performance Liquid Chromatography. Five kinds of polyphenols were detected and most of the impurity peaks were disappeared. This method can effectively purify the apple polyphenols and provide reference for large-scale industrial production of apple polyphenols.