54 strains of lactic acid bacteria( LAB) isolated from fermented dough were screened for the antimicrobial activity toward Listeria( L.) monocytogenes by dual-culture agar plate assay. After eliminating the effects of organic acid and hydrogen peroxide,the cell-free supernatant of one strain showed strong antimicrobial activity toward L. monocytogenes. Cell-free culture supernatants of the strain exhibited strong antimicrobial activity under acidic conditions. In addition,the inhibitory activity declined remarkably after digested by protease K and trypsin,and eliminated by heat treatment above 100 ℃. The result showed the inhibitory material which was metabolite of the strain had the features of protein,and could be classified as bacteriocin. The molecular weight of the bacteriocin was approximately 25 kDa by Tricine-SDS-PAGE assay. Based on phenotypic,biochemical tests and 16S rDNA sequence homology analysis,the strain were identified as Lactobacillus curvatus.