A method for determination of reducing carbohydrates in the green tea enzymatic hydrolysate by capillary zone electrophoresis( CZE) was established. Nine reducing carbohydrates which may be existed in the green tea enzymatic hydrolysate were derivatized with 1-phenyl-3-methyl-5-pyrazolone( PMP),and baseline separated by CZE under the condition of 200 mmol /L borate buffer pH 11. 0 and running voltage 25 kV at temperature 25 ℃. The standard curves of main reducing carbohydrate derivative in the green tea including maltose,lactose,xylose,arabinose,glucose,rhamnose,galactose,mannose and galacturonic acid showed good linearity in the range of 0. 5 ~ 10mmol /L and the R2value equivalent to 0. 9939 ~ 0. 9994. The minimum limit point of determination ranged from 0. 09to 0. 23 μmol /mL and quantitative recoveries of the nine reducing carbohydrates ranged from 94. 04% to 101. 9%.The optimized CZE method was found to be feasible to examine the compositions and content of reducing carbohydrates in the green tea extracts with enzyme treatment. The result showed that the enzymatic solution mainly contained five kinds of reducing carbohydrates including xylose,arabinose,glucose,galactose and galacturonic acid and their concentrations varied with different composite hydrolase source. The method for determination of reducing carbohydrates in the green tea enzymatic hydrolysate was characterized by easy operation,high sensitivity and good accuracy.